DAPI staining uses the fluorescent dye 4′,6-diamidino-2-phenylindole to bind strongly to A-T rich regions of DNA, producing bright blue fluorescence. It is widely used in microscopy to visualize nuclei in fixed or permeabilized cells, helping researchers study cell structure, count nuclei, and analyze apoptosis or cell cycle changes.
DAPI staining uses the fluorescent dye 4′,6-diamidino-2-phenylindole to bind strongly to A-T rich regions of DNA, producing bright blue fluorescence. It is widely used in microscopy to visualize nuclei in fixed or permeabilized cells, helping researchers study cell structure, count nuclei, and analyze apoptosis or cell cycle changes.